cd11c cre  (Jackson Laboratory)

Journal: Cell

Article Title: Distinct components of mRNA vaccines cooperate to instruct efficient germinal center responses

doi: 10.1016/j.cell.2025.11.023

Figure Lengend Snippet: (A) Representative expression of MHCII and CD11c post-injection of eGFP mRNA-LNP-DiI. Cells are pre-gated as Live, TCRβ − CD19 − . (B) Schematic of muscle ( Left ) and dLN ( Right ) photoactivation (PA) experiments. (C) Representative expression of RFP and Thy1.1 by DCs from dLNs of Muscle PA and dLN PA, 24 hours after injection. (D and E) Quantification of RFP + and RFP − Thy1.1 + DCs from C . PA cells are represented as red circles and non-PA cells as green circles. (F) Frequency of CD11b + (cDC2) from D and E . (G) Experimental design of panels H-J . (H) Representative frequencies of Tfh and GC B cells from G . (I-J) Quantification of frequency and number of Tfh cells ( I ) and GC B cells ( J ) from H . Mice were injected with 20 μg of eGFP mRNA-LNP-DiI ( A ), 30 μg Thy1.1 mRNA-LNP ( B-F ), and 30 μg rHA ( H-J ). In Group 1, rHA and eLNP were mixed and injected at the same time; in Group 2, eLNP were injected one hour before rHA at the same site; and in Group 3, eLNP and rHA were injected at the same time but in opposite limbs. In all experiments, n = 6-10 mice per group from 2 independent experiments. In panels D-F and I-J unpaired two-tailed Mann-Whitney U test was performed.

Article Snippet: Cd11c-cre -GFP mice: C57BL/6J-Tg( Itgax-cre ,-EGFP)4097Ach/J , The Jackson Laboratory , Cat#007567.

Techniques: Expressing, Injection, Two Tailed Test, MANN-WHITNEY